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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but thorough nucleotide sequence data just isn't still obtainable.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly contains the epitope recognized with the antibody.
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This guide outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Data Cyclin E belongs on the really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show unique expression and degradation styles which add on the temporal coordination of each and every mitotic more info function. Cyclin E forms a posh with and functions as being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates in the G1-S phase boundary which is degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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